Regulatory
Part:BBa_K3054003:Design
Designed by: Dharmik Hitesh Patel Group: iGEM19_Chalmers-Gothenburg (2019-10-20)
pTEF1/pCCW12 dual promoter (yeast)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 879
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 879
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 879
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 879
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 879
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 241
Design Notes
Single promoters for pCCW12 and pTEF1 were extracted from genome using PCR amplification. They were then attached to each other by fusion PCR using linker as complementary sequence for fusion
Source
Saccharomyces cerevisiae genome